Hung Lab

Deborah Hung, M.D./Ph.D.

Department of Molecular Biology
Simches Research Center
Boston, MA 02114

Phone: (617) 726-3117
Fax: (617) 726-6893
Email
Publications
    2012
  1. Grad YH, Lipsitch M, Griggs AD, Haas BJ, Shea TP, McCowan C, Montmayeur A, FitzGerald M, Wortman JR, Krogfelt KA, Bingen E, Weill FX, Tietze E, Flieger A, Lander ES, Nusbaum C, Birren BW, Hung DT, Hanage WP. Reply to Guy et al.: Support for a bottleneck in the 2011 Escherichia coli O104:H4 outbreak in Germany. Proc. Natl. Acad. Sci. U.S.A. 2012 Dec 26; 109(52):E3629-30.

  2. Chand NS, Clatworthy AE, Hung DT. The Two-Component Sensor KinB Acts as a Phosphatase To Regulate Pseudomonas aeruginosa Virulence. J. Bacteriol. 2012 Dec; 194(23):6537-47.

  3. McMahon TC, Stanley S, Kazyanskaya E, Hung D, Wood JL. The first synthesis of an epidiselenodiketopiperazine. Org. Lett. 2012 Sep 7; 14(17):4534-6.

  4. Stanley SA, Grant SS, Kawate T, Iwase N, Shimizu M, Wivagg C, Silvis M, Kazyanskaya E, Aquadro J, Golas A, Fitzgerald M, Dai H, Zhang L, Hung DT. Identification of Novel Inhibitors of M. tuberculosis Growth Using Whole Cell Based High-Throughput Screening. ACS Chem. Biol. 2012 Aug 17; 7(8):1377-84.

  5. Grant SS, Kaufmann BB, Chand NS, Haseley N, Hung DT. Eradication of bacterial persisters with antibiotic-generated hydroxyl radicals. Proc. Natl. Acad. Sci. U.S.A. 2012 Jul 24; 109(30):12147-52.

  6. Barczak AK, Gomez JE, Kaufmann BB, Hinson ER, Cosimi L, Borowsky ML, Onderdonk AB, Stanley SA, Kaur D, Bryant KF, Knipe DM, Sloutsky A, Hung DT. RNA signatures allow rapid identification of pathogens and antibiotic susceptibilities. Proc. Natl. Acad. Sci. U.S.A. 2012 Apr 17; 109(16):6217-22.

  7. Wivagg CN, Hung DT. Resuscitation-promoting factors are required for ?-lactam tolerance and the permeability barrier in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 2012 Mar; 56(3):1591-4.

  8. Grad YH, Lipsitch M, Feldgarden M, Arachchi HM, Cerqueira GC, Fitzgerald M, Godfrey P, Haas BJ, Murphy CI, Russ C, Sykes S, Walker BJ, Wortman JR, Young S, Zeng Q, Abouelleil A, Bochicchio J, Chauvin S, Desmet T, Gujja S, McCowan C, Montmayeur A, Steelman S, Frimodt-Møller J, Petersen AM, Struve C, Krogfelt KA, Bingen E, Weill FX, Lander ES, Nusbaum C, Birren BW, Hung DT, Hanage WP. Genomic epidemiology of the Escherichia coli O104:H4 outbreaks in Europe, 2011. Proc. Natl. Acad. Sci. U.S.A. 2012 Feb 21; 109(8):3065-70.

  9. Liu X, Bolla K, Ashforth EJ, Zhuo Y, Gao H, Huang P, Stanley SA, Hung DT, Zhang L. Systematics-guided bioprospecting for bioactive microbial natural products. Antonie Van Leeuwenhoek 2012 Jan; 101(1):55-66.

  10. Weiner B, Gomez J, Victor TC, Warren RM, Sloutsky A, Plikaytis BB, Posey JE, van Helden PD, Gey van Pittius NC, Koehrsen M, Sisk P, Stolte C, White J, Gagneux S, Birren B, Hung D, Murray M, Galagan J. Independent large scale duplications in multiple M. tuberculosis lineages overlapping the same genomic region. PLoS ONE 2012; 7(2):e26038.

  11. Chindelevitch L, Stanley S, Hung D, Regev A, Berger B. MetaMerge: scaling up genome-scale metabolic reconstructions with application to Mycobacterium tuberculosis. Genome Biol. 2012; 13(1):r6.

  12. 2011
  13. Chand NS, Hung DT. The two-component sensor kinase KinB acts as a non-canonical switch between acute and chronic infection. Virulence ; 2(6):553-8.

  14. Tamayo AG, Slater L, Taylor-Parker J, Bharti A, Harrison R, Hung DT, Murphy JR. GRP78(BiP) facilitates the cytosolic delivery of anthrax lethal factor (LF) in vivo and functions as an unfoldase in vitro. Mol. Microbiol. 2011 Sep; 81(5):1390-401.

  15. Chand NS, Lee JS, Clatworthy AE, Golas AJ, Smith RS, Hung DT. The sensor kinase KinB regulates virulence in acute Pseudomonas aeruginosa infection. J. Bacteriol. 2011 Jun; 193(12):2989-99.

  16. Gomez JE, Clatworthy A, Hung DT. Probing bacterial pathogenesis with genetics, genomics, and chemical biology: past, present, and future approaches. Crit. Rev. Biochem. Mol. Biol. 2011 Feb; 46(1):41-66.

  17. 2010
  18. Kaufmann BB, Hung DT. The fast track to multidrug resistance. Mol. Cell 2010 Feb 12; 37(3):297-8.

  19. 2009
  20. Hett EC, Hung DT. Targeting multiple biofilm pathways. Chem. Biol. 2009 Dec 24; 16(12):1216-8.

  21. Stanley SA, Hung DT. Toward a systems-level analysis of infection biology: a new method for conducting genetic screens in human cells. Sci Transl Med 2009 Dec 16; 1(11):11ps13.

  22. Barczak AK, Hung DT. Productive steps toward an antimicrobial targeting virulence. Curr. Opin. Microbiol. 2009 Oct; 12(5):490-6.

  23. Stanley SA, Hung DT. Chemical tools for dissecting bacterial physiology and virulence. Biochemistry 2009 Sep 22; 48(37):8776-86.

  24. Clatworthy AE, Lee JS, Leibman M, Kostun Z, Davidson AJ, Hung DT. Pseudomonas aeruginosa infection of zebrafish involves both host and pathogen determinants. Infect. Immun. 2009 Apr; 77(4):1293-303.

  25. Nusbaum C, Ohsumi TK, Gomez J, Aquadro J, Victor TC, Warren RM, Hung DT, Birren BW, Lander ES, Jaffe DB. Sensitive, specific polymorphism discovery in bacteria using massively parallel sequencing. Nat. Methods 2009 Jan; 6(1):67-9.

  26. 2007
  27. Clatworthy AE, Pierson E, Hung DT. Targeting virulence: a new paradigm for antimicrobial therapy. Nat. Chem. Biol. 2007 Sep; 3(9):541-8.

  28. Shakhnovich EA, Hung DT, Pierson E, Lee K, Mekalanos JJ. Virstatin inhibits dimerization of the transcriptional activator ToxT. Proc. Natl. Acad. Sci. U.S.A. 2007 Feb 13; 104(7):2372-7.

  29. 2006
  30. Hung DT, Rubin EJ. Chemical biology and bacteria: not simply a matter of life or death. Curr Opin Chem Biol 2006 Aug; 10(4):321-6.

  31. Hung DT, Zhu J, Sturtevant D, Mekalanos JJ. Bile acids stimulate biofilm formation in Vibrio cholerae. Mol. Microbiol. 2006 Jan; 59(1):193-201.

  32. 2005
  33. Hung DT, Shakhnovich EA, Pierson E, Mekalanos JJ. Small-molecule inhibitor of Vibrio cholerae virulence and intestinal colonization. Science 2005 Oct 28; 310(5748):670-4.

  34. Hung DT, Mekalanos JJ. Bile acids induce cholera toxin expression in Vibrio cholerae in a ToxT-independent manner. Proc. Natl. Acad. Sci. U.S.A. 2005 Feb 22; 102(8):3028-33.

  35. 2003
  36. Hung, D.T.; Lilly, C.M. Hemodynamic monitoring in the intensive care unit. Journal of Critical Illness. 2003; 18((5)):196-297.

  37. 2002
  38. Hung, D.T.; Baden L.R. A new era for sepsis treatment? Understanding sepsis as a consequence of host immune response. Expert Opin. Ther. Patents. 2002; 12((2), ):181-192.

  39. 1996
  40. Hung DT, Jamison TF, Schreiber SL. Understanding and controlling the cell cycle with natural products. Chem. Biol. 1996 Aug; 3(8):623-39.

  41. Hung DT, Chen J, Schreiber SL. (+)-Discodermolide binds to microtubules in stoichiometric ratio to tubulin dimers, blocks taxol binding and results in mitotic arrest. Chem. Biol. 1996 Apr; 3(4):287-93.

  42. Hung, D.T.; Nerenberg, J.B.; Schreiber, S.L. Syntheses of Discodermolides Useful for Investigating Microtubule Binding and Stabilization. J. Am. Chem. Soc. 1996; 118:11054-11080.

  43. Liang, J.; Hung, D.T.; Schreiber, S.L.; Clardy, J. Structure of the Human 25 kDa FK506 Binding Protein Complexed with Rapamycin. J. Am. Chem. Soc. 1996; 118:1231.

  44. 1994
  45. Hung DT, Nerenberg JB, Schreiber SL. Distinct binding and cellular properties of synthetic (+)- and (-)-discodermolides. Chem. Biol. 1994 Sep; 1(1):67-71.

  46. 1993
  47. Bram RJ, Hung DT, Martin PK, Schreiber SL, Crabtree GR. Identification of the immunophilins capable of mediating inhibition of signal transduction by cyclosporin A and FK506: roles of calcineurin binding and cellular location. Mol. Cell. Biol. 1993 Aug; 13(8):4760-9.

  48. Nerenberg, J.B.; Hung, D.T.; Schreiber, S.L. . Total synthesis of the Immunosuppressive Agent (-)-Discodermolide. J. Am. Chem. Soc.,. 1993; 115:12621.

  49. 1992
  50. Hung DT, Wong YH, Vu TK, Coughlin SR. The cloned platelet thrombin receptor couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cyclase. J. Biol. Chem. 1992 Oct 15; 267(29):20831-4.

  51. Scarborough RM, Naughton MA, Teng W, Hung DT, Rose J, Vu TK, Wheaton VI, Turck CW, Coughlin SR. Tethered ligand agonist peptides. Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function. J. Biol. Chem. 1992 Jul 5; 267(19):13146-9.

  52. Hung DT, Schreiber SL. cDNA cloning of a human 25 kDa FK506 and rapamycin binding protein. Biochem. Biophys. Res. Commun. 1992 Apr 30; 184(2):733-8.

  53. Garboczi DN, Hung DT, Wiley DC. HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides. Proc. Natl. Acad. Sci. U.S.A. 1992 Apr 15; 89(8):3429-33.

  54. Hung DT, Vu TH, Nelken NA, Coughlin SR. Thrombin-induced events in non-platelet cells are mediated by the unique proteolytic mechanism established for the cloned platelet thrombin receptor. J. Cell Biol. 1992 Feb; 116(3):827-32.

  55. Coughlin SR, Scarborough RM, Vu TK, Hung DT. Thrombin receptor structure and function. Cold Spring Harb. Symp. Quant. Biol. 1992; 57:149-54.

  56. 1991
  57. Vu TK, Hung DT, Wheaton VI, Coughlin SR. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 1991 Mar 22; 64(6):1057-68.

  58. 1987
  59. Evans, D.A.; Chapman, K.T.; Hung, D.T.; Kawaguchi, A.T. . Transition State ?-solvation by Aromatic Rings: An Electronic Contribution to Diels-Alder Reaction Diastereoselectivity. Angew. Chemie, Intl. Ed:. 1987; 26,:1184.

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