Clones & Mutants

Supported by:

NSF IOS-0618292, NSF2010: MAPK Cascade Signaling Networks


                     Summary

The six Excel files provide information for 97 plasmid DNA clones, 9 reporter genes and 88 MAPK cascade genes in plant expression vectors, including 58 MAPKKK (MTK), 10 MAPKK (MKK) and 20 MAPK (MPK) genes. All these clones (click on the clone information for details) have been deposited to ABRC and their sequences are available in GenBank (with GenBank ID). Some clones may carry polymorphisms, as we obtained most clones from Col but initially a few were from Ler (e.g., MKK clones).


1. 9 promoter-LUC or GFP reporter genes for diverse plant signaling pathways
2. 20 MPK clones
3. 10 MKK clones
4. 16 MTK A family (kinase domain) clones
5. 18 MTK B family (kinase domain) clones
6. 24 MTK C family (kinase domain) clones

One Word file provides the primer information for 8 mkk mutants, including mkk1, mkk2, mkk3, mkk4, mkk5, mkk6, mkk7, mkk9. We are bulking up the mkk mutant seeds to be donated to ABRC in the near future. Some of them are not true null mutants and one needs to be cautious in making conclusions.

 


1. 9 promoter-LUC or GFP reporter genes for diverse plant signaling pathways 

Excel file LUC and GFP reporter genes


2. 20 MPK clones

Excel file MPK clones


3. 10 MKK clones

Excel file MKK clones


4. 16 MTK A family (kinase domain) clones

Excel file MTK A family clones


5. 18 MTK B family (kinase domain) clones

Excel file MTK B famliy clones


6. 24 MTK C family (kinase domain) clones

Excel file MTK C family clones


Primer information

Word file with mkk primer information