Figure 1: Ligation of myc-RNA (RNA124) to the puromycin containingoligonucleotide. The reaction was performed as described above.The sequence of the phosphorylated puromycin containing linkerwas dA₂₁(C₉)₃dAdCdCPand the sequence of myc-RNA (RNA124) was the following:

A mixture of 5'-TTTTTTTTTTAGCGCAAGA and 5'-TTTTTTTTTTNAGCGCAAGAwas used as the splint. RNA124 was the starting material and RNA152was the ligated product.