Bruno Di Stefano

Hochedlinger Lab


Research Fellow


Email

Publications
    2019
  1. Tian TV, Di Stefano B, Stik G, Vila-Casadesús M, Sardina JL, Vidal E, Dasti A, Segura-Morales C, De Andrés-Aguayo L, Gómez A, Goldmann J, Jaenisch R, Graf T. Whsc1 links pluripotency exit with mesendoderm specification. Nat. Cell Biol. 2019 Jul; 21(7):824-834.

  2. Francesconi M, Di Stefano B, Berenguer C, de Andrés-Aguayo L, Plana-Carmona M, Mendez-Lago M, Guillaumet-Adkins A, Rodriguez-Esteban G, Gut M, Heyn H, Lehner B, Graf T. Single cell RNA-seq identifies the origins of heterogeneity in efficient cell transdifferentiation and reprogramming. Elife 2019 Mar 12; 8

  3. 2018
  4. Di Stefano B, Hochedlinger K. Novel Roles for SUMOylation in Cellular Plasticity. Trends Cell Biol. 2018 12; 28(12):971-973.

  5. Sardina JL, Collombet S, Tian TV, Gómez A, Di Stefano B, Berenguer C, Brumbaugh J, Stadhouders R, Segura-Morales C, Gut M, Heath S, Aranda S, Di Croce L, Hochedlinger K, Thieffry D, Graf T. Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate. Cell Stem Cell 2018 Sep 06.

  6. Di Stefano B, Ueda M, Sabri S, Brumbaugh J, Huebner AJ, Sahakyan A, Clement K, Clowers KJ, Erickson AR, Shioda K, Gygi SP, Gu H, Meissner A, Takashima Y, Plath K, Hochedlinger K. Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells. Nat. Methods 2018 Aug 20.

  7. Bar-Nur O, Gerli MFM, Di Stefano B, Almada AE, Galvin A, Coffey A, Huebner AJ, Feige P, Verheul C, Cheung P, Payzin-Dogru D, Paisant S, Anselmo A, Sadreyev RI, Ott HC, Tajbakhsh S, Rudnicki MA, Wagers AJ, Hochedlinger K. Direct Reprogramming of Mouse Fibroblasts into Functional Skeletal Muscle Progenitors. Stem Cell Reports 2018 May 08; 10(5):1505-1521.

  8. Stadhouders R, Vidal E, Serra F, Di Stefano B, Le Dily F, Quilez J, Gomez A, Collombet S, Berenguer C, Cuartero Y, Hecht J, Filion GJ, Beato M, Marti-Renom MA, Graf T. Transcription factors orchestrate dynamic interplay between genome topology and gene regulation during cell reprogramming. Nat. Genet. 2018 Jan 15.

  9. Massimino L, Flores-Garcia L, Di Stefano B, Colasante G, Icoresi-Mazzeo C, Zaghi M, Hamilton BA, Sessa A. TBR2 antagonizes retinoic acid dependent neuronal differentiation by repressing Zfp423 during corticogenesis. Dev. Biol. 2018 Jan 02.

  10. 2017
  11. Brumbaugh J, Di Stefano B, Wang X, Borkent M, Forouzmand E, Clowers KJ, Ji F, Schwarz BA, Kalocsay M, Elledge SJ, Chen Y, Sadreyev RI, Gygi SP, Hu G, Shi Y, Hochedlinger K. Nudt21 Controls Cell Fate by Connecting Alternative Polyadenylation to Chromatin Signaling. Cell 2017 Nov 30.

  12. Ruetz T, Pfisterer U, Di Stefano B, Ashmore J, Beniazza M, Tian TV, Kaemena DF, Tosti L, Tan W, Manning JR, Chantzoura E, Ottosson DR, Collombet S, Johnsson A, Cohen E, Yusa K, Linnarsson S, Graf T, Parmar M, Kaji K. Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming. Cell Stem Cell 2017 Nov 13.

  13. Choi J, Huebner AJ, Clement K, Walsh RM, Savol A, Lin K, Gu H, Di Stefano B, Brumbaugh J, Kim SY, Sharif J, Rose CM, Mohammad A, Odajima J, Charron J, Shioda T, Gnirke A, Gygi S, Koseki H, Sadreyev RI, Xiao A, Meissner A, Hochedlinger K. Prolonged Mek1/2 suppression impairs the developmental potential of embryonic stem cells. Nature 2017 Jul 26.

  14. Collombet S, van Oevelen C, Sardina Ortega JL, Abou-Jaoudé W, Di Stefano B, Thomas-Chollier M, Graf T, Thieffry D. Logical modeling of lymphoid and myeloid cell specification and transdifferentiation. Proc. Natl. Acad. Sci. U.S.A. 2017 Jun 06; 114(23):5792-5799.

  15. 2016
  16. Di Stefano B, Hochedlinger K. Cell reprogramming: Brain versus brawn. Nature 2016 Jun 16; 534(7607):332-3.

  17. Sardina JL, Di Stefano B, Graf T. How does C/EBPα speed up cell reprogramming? Cell Cycle 2016 Jun 7.

  18. Di Stefano B, Collombet S, Jakobsen JS, Wierer M, Sardina JL, Lackner A, Stadhouders R, Segura-Morales C, Francesconi M, Limone F, Mann M, Porse B, Thieffry D, Graf T. C/EBPα creates elite cells for iPSC reprogramming by upregulating Klf4 and increasing the levels of Lsd1 and Brd4. Nat. Cell Biol. 2016 Mar 14.

  19. Krijger PH, Di Stefano B, de Wit E, Limone F, van Oevelen C, de Laat W, Graf T. Cell-of-Origin-Specific 3D Genome Structure Acquired during Somatic Cell Reprogramming. Cell Stem Cell 2016 Mar 8.

  20. Di Stefano B, Graf T. Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse Pre-B Cells. Methods Mol. Biol. 2016; 1357:45-56.

  21. 2015
  22. Bueno C, Sardina JL, Di Stefano B, Romero-Moya D, Ariza L, Balanzategui A, Herreros A, Fraga MF, Granada I, Quintana-Bustamante O, Segovia JC, Nishimura K, Ohtaka M, Nakanishi M, Graf T, Menendez P. Reprogramming human B cells into induced pluripotent stem cells and its enhancement by C/EBP?. Leukemia 2015 Oct 26.

  23. 2014
  24. Di Stefano B, Graf T. Hi-TEC reprogramming for organ regeneration. Nat. Cell Biol. 2014 Sep; 16(9):824-5.

  25. Di Stefano B, Sardina JL, van Oevelen C, Collombet S, Kallin EM, Vicent GP, Lu J, Thieffry D, Beato M, Graf T. C/EBP? poises B cells for rapid reprogramming into induced pluripotent stem cells. Nature 2014 Feb 13; 506(7487):235-9.

  26. Aloia L, Di Stefano B, Sessa A, Morey L, Santanach A, Gutierrez A, Cozzuto L, Benitah SA, Graf T, Broccoli V, Di Croce L. Zrf1 is required to establish and maintain neural progenitor identity. Genes Dev. 2014 Jan 15; 28(2):182-97.

  27. Di Stefano B, Collombet S, Graf T. Time-resolved gene expression profiling during reprogramming of C/EBP?-pulsed B cells into iPS cells. Sci Data 2014; 1:140008.

  28. 2013
  29. Aloia L, Di Stefano B, Di Croce L. Polycomb complexes in stem cells and embryonic development. Development 2013 Jun; 140(12):2525-34.

  30. 2011
  31. Di Stefano B, Maffioletti SM, Gentner B, Ungaro F, Schira G, Naldini L, Broccoli V. A microRNA-based system for selecting and maintaining the pluripotent state in human induced pluripotent stem cells. Stem Cells 2011 Nov; 29(11):1684-95.

  32. Verpelli C, Dvoretskova E, Vicidomini C, Rossi F, Chiappalone M, Schoen M, Di Stefano B, Mantegazza R, Broccoli V, Dityatev A, Sala C. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. J. Biol. Chem. 2011 Oct 7; 286(40):34839-50.

  33. Lombardo A, Cesana D, Genovese P, Di Stefano B, Provasi E, Colombo DF, Neri M, Magnani Z, Cantore A, Lo Riso P, Damo M, Pello OM, Holmes MC, Gregory PD, Gritti A, Broccoli V, Bonini C, Naldini L. Site-specific integration and tailoring of cassette design for sustainable gene transfer. Nat. Methods 2011; 8(10):861-9.

  34. 2010
  35. Di Stefano B, Buecker C, Ungaro F, Prigione A, Chen HH, Welling M, Eijpe M, Mostoslavsky G, Tesar P, Adjaye J, Geijsen N, Broccoli V. An ES-like pluripotent state in FGF-dependent murine iPS cells. PLoS ONE 2010; 5(12):e16092.

  36. 2009
  37. Di Stefano B, Prigione A, Broccoli V. Efficient genetic reprogramming of unmodified somatic neural progenitors uncovers the essential requirement of Oct4 and Klf4. Stem Cells Dev. 2009 Jun; 18(5):707-16.

  38. 2008
  39. Fiorio E, Mercanti A, Terrasi M, Micciolo R, Remo A, Auriemma A, Molino A, Parolin V, Di Stefano B, Bonetti F, Giordano A, Cetto GL, Surmacz E. Leptin/HER2 crosstalk in breast cancer: in vitro study and preliminary in vivo analysis. BMC Cancer 2008; 8:305.

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