Sheen Lab

Jen Sheen, Ph.D.

Department of Molecular Biology
Simches Research Center
Boston, MA 02114

Phone: (617) 726-5916
Fax: (617) 726-6893

  1. Xiong Y, McCormack M, Li L, Hall Q, Xiang C, Sheen J. Glucose-TOR signalling reprograms the transcriptome and activates meristems. Nature 2013 Apr 11; 496(7444):181-6.

  2. Boudsocq M, Sheen J. CDPKs in immune and stress signaling. Trends Plant Sci. 2013 Jan; 18(1):30-40.

  3. Gao X, Chen X, Lin W, Lu D, Niu Y, Li L, Cheng C, McCormack M, Sheen J, Shan L, He P. Bifurcation of Arabidopsis NLR immune signaling via Ca²?-dependent protein kinases. PLoS Pathog. 2013 Jan; 9(1):e1003127.

  4. 2012
  5. Lee H, Khatri A, Plotnikov JM, Zhang XC, Sheen J. Complexity in differential peptide-receptor signaling: response to Segonzac et Al. and Mueller et Al. commentaries. Plant Cell 2012 Aug; 24(8):3177-85.

  6. Hwang I, Sheen J, Müller B. Cytokinin signaling networks. Annu Rev Plant Biol 2012 Jun 2; 63:353-80.

  7. Sheen J. An interview with Jen Sheen. Trends Plant Sci. 2012 Jun; 17(6):321-2.

  8. Xiong Y, Sheen J. Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. J. Biol. Chem. 2012 Jan 20; 287(4):2836-42.

  9. Li JF, Sheen J. DNA purification from multiple sources in plant research with homemade silica resins. Methods Mol. Biol. 2012; 862:53-9.

  10. Liu KH, McCormack M, Sheen J. Targeted parallel sequencing of large genetically-defined genomic regions for identifying mutations in Arabidopsis. Plant Methods 2012; 8:12.

  11. Niu Y, Sheen J. Transient expression assays for quantifying signaling output. Methods Mol. Biol. 2012; 876:195-206.

  12. 2011
  13. Hamel LP, Benchabane M, Nicole MC, Major IT, Morency MJ, Pelletier G, Beaudoin N, Sheen J, Séguin A. Stress-responsive mitogen-activated protein kinases interact with the EAR motif of a poplar zinc finger protein and mediate its degradation through the 26S proteasome. Plant Physiol. 2011 Nov; 157(3):1379-93.

  14. Tena G, Boudsocq M, Sheen J. Protein kinase signaling networks in plant innate immunity. Curr. Opin. Plant Biol. 2011 Oct; 14(5):519-29.

  15. Lee H, Chah OK, Sheen J. Stem-cell-triggered immunity through CLV3p-FLS2 signalling. Nature 2011 May 19; 473(7347):376-9.

  16. 2010
  17. Sheen J. Discover and connect cellular signaling. Plant Physiol. 2010 Oct; 154(2):562-6.

  18. Boudsocq M, Willmann MR, McCormack M, Lee H, Shan L, He P, Bush J, Cheng SH, Sheen J. Differential innate immune signalling via Ca(2+) sensor protein kinases. Nature 2010 Mar 18; 464(7287):418-22.

  19. Cho YH, Sheen J, Yoo SD. Low glucose uncouples hexokinase1-dependent sugar signaling from stress and defense hormone abscisic acid and C2H4 responses in Arabidopsis. Plant Physiol. 2010 Mar; 152(3):1180-2.

  20. Li JF, Sheen J. Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology. Plant Methods 2010; 6(1):1.

  21. Boudsocq M, Sheen J. . Calcium sensing and signaling. In: Abiotic Stress Adaptation in Plants: Physiological, Molecular and Genomic Foundation. 2010; 75-90.

  22. 2009
  23. Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu JK. In vitro reconstitution of an abscisic acid signalling pathway. Nature 2009 Dec 3; 462(7273):660-4.

  24. Yoo SD, Cho Y, Sheen J. Emerging connections in the ethylene signaling network. Trends Plant Sci. 2009 May; 14(5):270-9.

  25. Cho JI, Ryoo N, Eom JS, Lee DW, Kim HB, Jeong SW, Kwon YK, Bhoo SH, Hahn TR, Park YI, Hwang I, Sheen J, Jeon JS. Role of the rice hexokinases OsHXK5 and OsHXK6 as glucose sensors. Plant Physiol. 2009 Feb; 149(2):745-59.

  26. 2008
  27. Yoo SD, Sheen J. MAPK signaling in plant hormone ethylene signal transduction. Plant Signal Behav 2008 Oct; 3(10):848-9.

  28. Baena-González E, Sheen J. Convergent energy and stress signaling. Trends Plant Sci. 2008 Sep; 13(9):474-82.

  29. Karve A, Rauh BL, Xia X, Kandasamy M, Meagher RB, Sheen J, Moore BD. Expression and evolutionary features of the hexokinase gene family in Arabidopsis. Planta 2008 Aug; 228(3):411-25.

  30. Shan L, He P, Li J, Heese A, Peck SC, Nürnberger T, Martin GB, Sheen J. Bacterial effectors target the common signaling partner BAK1 to disrupt multiple MAMP receptor-signaling complexes and impede plant immunity. Cell Host Microbe 2008 Jul 17; 4(1):17-27.

  31. Müller B, Sheen J. Cytokinin and auxin interaction in root stem-cell specification during early embryogenesis. Nature 2008 Jun 19; 453(7198):1094-7.

  32. Yoo SD, Cho YH, Tena G, Xiong Y, Sheen J. Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling. Nature 2008 Feb 14; 451(7180):789-95.

  33. Ramon M, Rolland F, Sheen J. Sugar sensing and signaling. Arabidopsis Book 2008; 6:e0117.

  34. Sheen J, He P, Shan L, Xiong Y, Tena G, Yoo SD, Cho YH, Boudsocq M, Lee H. . Signaling specificity and complexity of MAPK cascades in plant innate immunity. Biology of Molecular Plant-Microbe Interactions. 2008; 86:1-10.

  35. Ramon M, Rolland F, Sheen J. . Sugar sensing and signaling in Arabidopsis. The Arabidopsis Book. 2008.

  36. Baena-González E, Rolland F, Sheen J. . KIN10/11 are master regulators of the convergent stress transcriptome. In: Photosynthesis. Energy from the Sun: 14th International Congress on Photosynthesis. 2008; 1337-1344.

  37. 2007
  38. Chen Z, Agnew JL, Cohen JD, He P, Shan L, Sheen J, Kunkel BN. Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology. Proc. Natl. Acad. Sci. U.S.A. 2007 Dec 11; 104(50):20131-6.

  39. Xiao F, He P, Abramovitch RB, Dawson JE, Nicholson LK, Sheen J, Martin GB. The N-terminal region of Pseudomonas type III effector AvrPtoB elicits Pto-dependent immunity and has two distinct virulence determinants. Plant J. 2007 Nov; 52(4):595-614.

  40. Müller B, Sheen J. Cytokinin signaling pathway. Sci. STKE 2007 Oct 9; 2007(407):cm4.

  41. Müller B, Sheen J. Arabidopsis cytokinin signaling pathway. Sci. STKE 2007 Oct 9; 2007(407):cm5.

  42. Müller B, Sheen J. Advances in cytokinin signaling. Science 2007 Oct 5; 318(5847):68-9.

  43. Baena-González E, Rolland F, Thevelein JM, Sheen J. A central integrator of transcription networks in plant stress and energy signalling. Nature 2007 Aug 23; 448(7156):938-42.

  44. He P, Shan L, Sheen J. Elicitation and suppression of microbe-associated molecular pattern-triggered immunity in plant-microbe interactions. Cell. Microbiol. 2007 Jun; 9(6):1385-96.

  45. Shan L, He P, Sheen J. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host Microbe 2007 May 17; 1(3):167-74.

  46. Sheen J, He P. Nuclear actions in innate immune signaling. Cell 2007 Mar 9; 128(5):821-3.

  47. Cho YH, Yoo SD, Sheen J. Glucose signaling through nuclear hexokinase1 complex in Arabidopsis. Plant Signal Behav 2007 Mar; 2(2):123-4.

  48. He P, Shan L, Sheen J. The use of protoplasts to study innate immune responses. Methods Mol. Biol. 2007; 354:1-9.

  49. Yoo SD, Cho YH, Sheen J. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc 2007; 2(7):1565-72.

  50. He P, Shan L, Sheen J. Endless Hide-And-Seek: Dynamic Coevolution in Plant-Bacterium Warfare. J. Int. Plant Biol. 2007; 49:105-111.

  51. 2006
  52. Cho YH, Yoo SD, Sheen J. Regulatory functions of nuclear hexokinase1 complex in glucose signaling. Cell 2006 Nov 3; 127(3):579-89.

  53. He P, Shan L, Lin NC, Martin GB, Kemmerling B, Nürnberger T, Sheen J. Specific bacterial suppressors of MAMP signaling upstream of MAPKKK in Arabidopsis innate immunity. Cell 2006 May 5; 125(3):563-75.

  54. Hamel LP, Nicole MC, Sritubtim S, Morency MJ, Ellis M, Ehlting J, Beaudoin N, Barbazuk B, Klessig D, Lee J, Martin G, Mundy J, Ohashi Y, Scheel D, Sheen J, Xing T, Zhang S, Seguin A. Ancient signals: comparative genomics of plant MAPK and MAPKK gene families. Trends Plant Sci. 2006 Apr; 11(4):192-8.

  55. Kim HJ, Ryu H, Hong SH, Woo HR, Lim PO, Lee IC, Sheen J, Nam HG, Hwang I. Cytokinin-mediated control of leaf longevity by AHK3 through phosphorylation of ARR2 in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 2006 Jan 17; 103(3):814-9.

  56. Rolland F, Baena-Gonzalez E, Sheen J. Sugar sensing and signaling in plants: conserved and novel mechanisms. Annu Rev Plant Biol 2006; 57:675-709.

  57. 2005
  58. Rolland F, Sheen J. Sugar sensing and signalling networks in plants. Biochem. Soc. Trans. 2005 Feb; 33(Pt 1):269-71.

  59. Rolland F, Sheen J . Sugar sensing and signaling networks in plants. Biochemical Society Transactions. 2005; 33:269-271.

  60. 2004
  61. Shou H, Bordallo P, Fan JB, Yeakley JM, Bibikova M, Sheen J, Wang K. Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize. Proc. Natl. Acad. Sci. U.S.A. 2004 Mar 2; 101(9):3298-303.

  62. Sheen J, Kay S. Exploring new functions and actions of regulatory molecules. Curr. Opin. Plant Biol. 2004; 7:487-490.

  63. 2003
  64. Yanagisawa S, Yoo SD, Sheen J. Differential regulation of EIN3 stability by glucose and ethylene signalling in plants. Nature 2003 Oct 2; 425(6957):521-5.

  65. Moore B, Zhou L, Rolland F, Hall Q, Cheng WH, Liu YX, Hwang I, Jones T, Sheen J. Role of the Arabidopsis glucose sensor HXK1 in nutrient, light, and hormonal signaling. Science 2003 Apr 11; 300(5617):332-6.

  66. León P, Sheen J. Sugar and hormone connections. Trends Plant Sci. 2003 Mar; 8(3):110-6.

  67. 2002
  68. Cheng WH, Endo A, Zhou L, Penney J, Chen HC, Arroyo A, Leon P, Nambara E, Asami T, Seo M, Koshiba T, Sheen J. A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions. Plant Cell 2002 Nov; 14(11):2723-43.

  69. Cheng SH, Willmann MR, Chen HC, Sheen J. Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol. 2002 Jun; 129(2):469-85.

  70. Hwang I, Chen HC, Sheen J. Two-component signal transduction pathways in Arabidopsis. Plant Physiol. 2002 Jun; 129(2):500-15.

  71. Sheen J. Phosphorelay and transcription control in cytokinin signal transduction. Science 2002 May 31; 296(5573):1650-2.

  72. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 2002 Feb 28; 415(6875):977-83.

  73. Rolland F, Moore B, Sheen J. Sugar sensing and signaling in plants. Plant Cell 2002; 14 Suppl:S185-205.

  74. 2001
  75. Sheen J. Signal transduction in maize and Arabidopsis mesophyll protoplasts. Plant Physiol. 2001 Dec; 127(4):1466-75.

  76. Shen Q, Gomez-Cadenas A, Zhang P, Walker-Simmons MK, Sheen J, Ho TH. Dissection of abscisic acid signal transduction pathways in barley aleurone layers. Plant Mol. Biol. 2001 Oct; 47(3):437-48.

  77. Tena G, Asai T, Chiu WL, Sheen J. Plant mitogen-activated protein kinase signaling cascades. Curr. Opin. Plant Biol. 2001 Oct; 4(5):392-400.

  78. Hwang I, Sheen J. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 2001 Sep 27; 413(6854):383-9.

  79. Cheng SH, Sheen J, Gerrish C, Bolwell GP. Molecular identification of phenylalanine ammonia-lyase as a substrate of a specific constitutively active Arabidopsis CDPK expressed in maize protoplasts. FEBS Lett. 2001 Aug 17; 503(2-3):185-8.

  80. Seidman CE, Struhl K, Sheen J, Jessen T. Introduction of plasmid DNA into cells. Curr Protoc Mol Biol 2001 May; Chapter 1:Unit1.8.

  81. Kingston RE, Sheen J, Moore D. Isotopic assays for reporter gene activity. Curr Protoc Mol Biol 2001 May; Chapter 9:Unit9.7A.

  82. Kingston RE, Sheen J, Moore D. Isotopic assays for reporter gene activity. Curr Protoc Pharmacol 2001 May; Chapter 6:Unit 6.4.

  83. Kausch AP, Owen TP, Zachwieja SJ, Flynn AR, Sheen J. Mesophyll-specific, light and metabolic regulation of the C4 PPCZm1 promoter in transgenic maize. Plant Mol. Biol. 2001 Jan; 45(1):1-15.

  84. 2000
  85. Xiao W, Sheen J, Jang JC. The role of hexokinase in plant sugar signal transduction and growth and development. Plant Mol. Biol. 2000 Nov; 44(4):451-61.

  86. Asai T, Stone JM, Heard JE, Kovtun Y, Yorgey P, Sheen J, Ausubel FM. Fumonisin B1-induced cell death in arabidopsis protoplasts requires jasmonate-, ethylene-, and salicylate-dependent signaling pathways. Plant Cell 2000 Oct; 12(10):1823-36.

  87. Arenas-Huertero F, Arroyo A, Zhou L, Sheen J, León P. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes Dev. 2000 Aug 15; 14(16):2085-96.

  88. Jang JC, Fujioka S, Tasaka M, Seto H, Takatsuto S, Ishii A, Aida M, Yoshida S, Sheen J. A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana. Genes Dev. 2000 Jun 15; 14(12):1485-97.

  89. Kovtun Y, Chiu WL, Tena G, Sheen J. Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants. Proc. Natl. Acad. Sci. U.S.A. 2000 Mar 14; 97(6):2940-5.

  90. 1999
  91. Sheen J, Zhou L, Jang JC. Sugars as signaling molecules. Curr. Opin. Plant Biol. 1999 Oct; 2(5):410-8.

  92. Moore Bd, Sheen J. Plant sugar sensing and signaling - a complex reality. Trends Plant Sci. 1999 Jul; 4(7):250.

  93. Sheen J. C4 GENE EXPRESSION. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1999 Jun; 50:187-217.

  94. Chang CC, Sheen J, Bligny M, Niwa Y, Lerbs-Mache S, Stern DB. Functional analysis of two maize cDNAs encoding T7-like RNA polymerases. Plant Cell 1999 May; 11(5):911-26.

  95. Sheen J, Zhou L, Jang JC . Sugars as signaling molecules. Curr. Opin. Plant Biol. 1999; 2:410-418.

  96. 1998
  97. Kovtun Y, Chiu WL, Zeng W, Sheen J. Suppression of auxin signal transduction by a MAPK cascade in higher plants. Nature 1998 Oct 15; 395(6703):716-20.

  98. Zhou L, Jang JC, Jones TL, Sheen J. Glucose and ethylene signal transduction crosstalk revealed by an Arabidopsis glucose-insensitive mutant. Proc. Natl. Acad. Sci. U.S.A. 1998 Aug 18; 95(17):10294-9.

  99. Sheen J. Mutational analysis of protein phosphatase 2C involved in abscisic acid signal transduction in higher plants. Proc. Natl. Acad. Sci. U.S.A. 1998 Feb 3; 95(3):975-80.

  100. Yanagisawa S, Sheen J. Involvement of maize Dof zinc finger proteins in tissue-specific and light-regulated gene expression. Plant Cell 1998 Jan; 10(1):75-89.

  101. 1997
  102. Saijo Y, Hata S, Sheen J, Izui K. cDNA cloning and prokaryotic expression of maize calcium-dependent protein kinases. Biochim. Biophys. Acta 1997 Feb 7; 1350(2):109-14.

  103. Jang JC, León P, Zhou L, Sheen J. Hexokinase as a sugar sensor in higher plants. Plant Cell 1997 Jan; 9(1):5-19.

  104. Jang JC, Sheen J . Sugar sensing in higher plants. Trends in Plant Science. 1997; 2:208-214.

  105. 1996
  106. Sheen J. Ca2+-dependent protein kinases and stress signal transduction in plants. Science 1996 Dec 13; 274(5294):1900-2.

  107. Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J. Engineered GFP as a vital reporter in plants. Curr. Biol. 1996 Mar 1; 6(3):325-30.

  108. 1995
  109. Sheen J, Hwang S, Niwa Y, Kobayashi H, Galbraith DW. Green-fluorescent protein as a new vital marker in plant cells. Plant J. 1995 Nov; 8(5):777-84.

  110. Sheen J. Methods for mesophyll and bundle sheath cell separation. Methods Cell Biol. 1995; 49:305-14.

  111. Galbraith DW, Lambert GM, Grebenok RJ, Sheen J. Flow cytometric analysis of transgene expression in higher plants: green-fluorescent protein. Methods Cell Biol. 1995; 50:3-14.

  112. 1994
  113. Jang JC, Sheen J. Sugar sensing in higher plants. Plant Cell 1994 Nov; 6(11):1665-79.

  114. Sheen J. Feedback control of gene expression. Photosyntheis Reseach. 1994; 39:427-438.

  115. Sheen J. Sugar and acetate repression of photosynthetic gene transcription. In US-Japan Binational Seminar on Environmental Stress and Photosynthesis, Physiological and Molecular Approaches. 1994; 53-56.

  116. 1993
  117. Sheen J. Protein phosphatase activity is required for light-inducible gene expression in maize. EMBO J. 1993 Sep; 12(9):3497-505.

  118. 1992
  119. Schäffner AR, Sheen J. Maize C4 photosynthesis involves differential regulation of phosphoenolpyruvate carboxylase genes. Plant J. 1992 Mar; 2(2):221-32.

  120. Sheen J, Huang H, Schäffner AR, Leon P, Jang JC. . Sugars, fatty acids, and photosynthetic gene expression. In Research in Photosynthesis IX. 1992; 753-760.

  121. 1991
  122. Schäffner AR, Sheen J. Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters. Plant Cell 1991 Sep; 3(9):997-1012.

  123. Sheen J. Molecular mechanisms underlying the differential expression of maize pyruvate, orthophosphate dikinase genes. Plant Cell 1991 Mar; 3(3):225-45.

  124. 1990
  125. Sheen J. Metabolic repression of transcription in higher plants. Plant Cell 1990 Oct; 2(10):1027-38.

  126. 1989
  127. Sheen J. A simple phase-extraction assay for CAT activity. In Current Protocols in Molecular Biology. 1989; 9:5-8.

  128. 1988
  129. Sheen JY, Seed B. Electrolyte gradient gels for DNA sequencing. BioTechniques ; 6(10):942-4.

  130. Seed B, Sheen JY. A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene 1988 Jul 30; 67(2):271-7.

  131. Sheen JY, Bogorad L. Differential Expression in Bundle Sheath and Mesophyll Cells of Maize of Genes for Photosystem II Components Encoded by the Plastid Genome. Plant Physiol. 1988 Apr; 86(4):1020-6.

  132. Sheen J. High-efficiency transformation by electroporation. In Current Protocols in Molecular Biology. 1988; 8:4-8.

  133. 1987
  134. Sheen JY, Bogorad L. Differential expression of C4 pathway genes in mesophyll and bundle sheath cells of greening maize leaves. J. Biol. Chem. 1987 Aug 25; 262(24):11726-30.

  135. 1986
  136. Sheen JY, Bogorad L. Expression of the ribulose-1,5-bisphosphate carboxylase large subunit gene and three small subunit genes in two cell types of maize leaves. EMBO J. 1986 Dec 20; 5(13):3417-22.

  137. Sheen JY, Bogorad L. Differential expression of six light-harvesting chlorophyll a/b binding protein genes in maize leaf cell types. Proc. Natl. Acad. Sci. U.S.A. 1986 Oct; 83(20):7811-5.

  138. 1985
  139. Sheen JY, Bogorad L. Differential expression of the ribulose bisphosphate carboxylase large subunit gene in bundle sheath and mesophyll cells of developing maize leaves is influenced by light. Plant Physiol. 1985 Dec; 79(4):1072-6.

  140. 1983
  141. Goldberg ML, Sheen JY, Gehring WJ, Green MM. Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome. Proc. Natl. Acad. Sci. U.S.A. 1983 Aug; 80(16):5017-21.

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