Table I  Characteristics of Arabidopsis CDPKs.										
 Namea (previous name)	 AGI IDb	 Genomic 	   Expressed	Mol wt (kDa) 	V.D.e	 EFf	N-term.	   N-	  Subcel.i 	Notej
		 LocusC	     Gene?d				a. a.g 	 Myrist.h 	   Dist.	
CPK1(AK1)	At5g04870	MUK11.19	     3 EST	68.2	149	4	MGNTCVGP	  Yes	 peroxisome	1
CPK2	At3g10660	F13M14.5	     4 EST	72.2	185	4	MGNACVGP	  Yes	     ER	2
CPK3(6)	At4g23650	F9D16.120	     9 EST	59.3	77	4	NGHRHSKS	  No	     ?	" 2,3"
CPK4	At4g09570	T25P22.10	     2 EST	56.4	24	4	MEKPNPRR	  No	     ?	2
CPK5	At4g35310	F23E12130	     7 EST	62.1	96	4	MGNSCRGS	  Yes	  membrane	2
CPK6(3)	At2g17290	F5J6.13	     4 EST	61.6	84	4	MGNSCRGS	  Yes	  membrane	2
CPK7	At5g12480		      Yes	60.3	58	3	MGNCCGNP	  Yes	     ?	2
CPK8(19)	At5g19450	F7K24.200	     6 EST	59.9	56	3	MGNCCASP	  Yes	     ?	" 2,3"
CPK9	At3g20410	MQC12.17	     8 EST	60.4	90	4	MGNCFAKN	  Yes	     ?	2
CPK10(1)	At1g18890	F6A14.1	     2 EST	61.4	62	3	MGNCNACV	  Yes	     ?	4
CPK11(2)	At1g35670	F15O4.8	      Yes	55.9	26	4	METKPNPR	  No	     ?	4
CPK12(9)	At5g23580	MQM1.15	      Yes	55.4	21	4	MANKPRTR	  No	     ?	5
CPK13	At3g51850	ORF10	      Yes	59.4	53	2	MGNCCRSP	  No	     ?	5
CPK14	At2g41860	T6D20.24	      ?	60.1	53	3	MGNCCGTA	  Yes	     ?	
CPK15	At4g21940	T8O5.150	      Yes	62.6	101	4	MGCFSSKH	  Yes	     ?	6
CPK16	At2g17890	T13L16.9	     1 EST	64.8	107	4	MGLCFSSA	  Yes	     ?	6
CPK17	At5g12180	MXC9.14	RAFL06-70-F18	58.5	72	4	MGNCCSHG	  Yes	     ?	
CPK18	At4g36070	T19K4.200	      Yes	60.2	70	4	MGLCFSSP	  Yes	     ?	
CPK19	At1g61950	F8K4.26	      Yes	62.9	97	3	MGCLCINL	  No	     ?	5
CPK20	At2g38910	T7F6.8	     X86963	64.7	133	4	MGNTCVGP	  Yes	     ?	
CPK21	At4g04720	T4B21.13	     5 EST	59.9	79	4	MGCFSSKH	  Yes	     ?	6
CPK22	At4g04710	T4B21.12	     3 EST	55.9	35	5	MGNCCGSK	  Yes	     ?	
CPK23	At4g04740	T4B21.15	     2 EST	58.7	68	3	MGCFSSKH	  Yes	     ?	
CPK24	At2g31500	T9H9.2	      ?	66.2	65	4	MGSCVSSP	  Yes	     ?	
CPK25	At2g35890	F11F19.20	      Yes	58.9	131	1	MGNVCVHM	  Yes	     ?	5
CPK26	At4g38230	F20D10.350	      ?	54.3	23	4	MKHSGGNQ	  No	     ?	
CPK27	At4g04700	T4B21.11	      Yes	54.9	27	0	MGCFSSKE	  Yes	     ?	
CPK28	At5g66210	K2A18.29	     5 EST	59	61	4	MGVCFSAI	  Yes	     ?	
CPK29	At1g76040	T4O12.25	     1 EST	60.5	84	3	MGCYSSKN	  No	     ?	
CPK30(1a)	At1g74740	F25A4.29	     2 EST	64.2	58	4	MGNCIACV	  Yes	     ?	
CPK31	At4g04695	T19J18.7	      ?	51.4	47	4	MMILHKPP	  No	     ?	
CPK32	At3g57530	T8H10.130	     7 EST	63.4	51	3	MGNCCGTA	  Yes	     ?	
CPK33	At1g50700	F17J6.22	      Yes	58.6	57	4	MGNCLAKK	  Yes	     ?	
CPK34	At5g19360	F7K24.110	      Yes	58.2	49	4	MGNCCSHG	  Yes	     ?	5
"a Numbers in parentheses indicate names given to previously published Arabidopsis CDPKs.  6, CDPK6; 3, CDPK3, 19, CDPK19; 1, CDPK1; 2, CDPK2; 9, CDPK9; 1a, CDPK1a.   b Systematic designation given to gene by Arabidopsis Genome Inititive.  Genes marked by a asterisk were not assigned numbers during annotation.   c Designation of gene locus on annotated bacterial artificial chromosome.  D Indicates whether gene is expressed, based on cloned cDNA (see Note for reference) or presence of cognated EST or full length cDNA.  e Numbers of amino acids in variable domain.  f Number of EF hands predicted by SMART (Shultz et al., 1998) and by sequence aligment (Fig. 2).   g The first 8 amino acids at N-terminus of the CDPKs.  hThe prediction of Myristoylation motif is based on PROSITE program (Hoffmann et al., 1999) with considerations drew from Towler et al. (1988), Ellard-Ivey et al. (1999), Martin and Busconi (2000), Lu et al.,(2001), and Raices et al. (2001).  i Subcellular distribution.   j 1, Harper et al., 1993; 2, Hrabak et al., 1996; 3, Hong et al., 1996; 4, Urao et al., 1994; 5, Willmann et al., unpublished; 6, Harmon et al., 2001."