David Wilson

4/12/98 

dNTP stocks: We buy the solid salts from Sigma, dissolve themin water, and neutralize them. These stocks are stored on the bottom shelfof the verticle -80 freezer.

Here is a table of the catalog number of each Sigma dNTP, the volumerequired to give a 20mM dNTP solution from 1g of solid, and the approximatevolume of NaOH required to neutralize the solution. Solutions are broughtto a pH of 7-8, as measure by pH paper.

catalog # dNTP final volume for approx. vol 50% 20mM sol'n NaOH added

T0251 TTP 90.7 mL 45 uL

D4010 dGTP 86.7 mL 43 uL

D6500 dATP 93.4 mL 168 uL

D4635 dCTP 97.8 mL 175 uL

NTP stocks:

We buy these as solid salts from Pharmacia. The table below lists thevolume required to give a 100mM NTP solution from 1g of solid, and theapproximate volume of NaOH required to neutralize the solution. Solutionsare brought to a pH of 7-8, as measure by pH paper.

catalog # NTP final volume for approx. vol 50% 100mM sol'n NaOH added

27-2000 GTP 16.4 mL 81 uL

27-1006-01 ATP 16.6 mL 178 uL

28-0700-03 UTP 17.1 mL 79 uL

27-1200 CTP 17.1 mL 167 uL