Agarose loading dyesare used to quantitate amount of DNA present as well as providing a molecularweight standards. Quantitation markers will not be prepared but dilutedand used by others.
Order 100 bp DNA markerand Low MW Mass Ladder from Gibco BRL.
100bp DNA markerpreparation:
to make 7.5 ml
750 ul 100 bp DNA ladder(3 tubes/250ul each)
150 ul 10% SDS (2%final)
500 ul Bromphenol Blueloading dye
1000 ul Orange loadingdye
50 ul 3M NaCl
5050 ul H20
Make 750 ul aliquotesand store at -20 C.
100 bp Ladder LowDNA Mass Ladder
Note: 10 ul of the100 bp ladder has these corresponding masses.
100 = 30ng
200 = 45ng
300 = 45ng