Jen Sheen


Department of Genetics
Harvard Medical School
Department of Molecular Biology
185 Cambridge St. - CPZN7624E
Massachusetts General Hospital
Boston, MA 02114
Office: 617 726-5916
Lab:     617 726-5963
Fax:     617 726-6893


We are probing plant life by developing simple and powerful tools and strategies to unravel plant signal transduction pathways extending from sensors/receptors to signaling cascades and target genes and proteins that are central to energy and metabolic homeostasis, innate immunity, stress adaptation, cell fate specification, plant shape and architecture determination. Our investigations are guided by our curiosity and the desire to promote the use of green plants as a versatile and fascinating model system for discovering fundamental principles in the regulatory networks of living organisms.

HMS Course Genetics 369 Molecular Mechanisms of Plant Signal Transduction
Genetics 300 Molecular mechanisms of plant signal transduction (Lectures & seminars)
Genetics 369 Molecular mechanisms of plant signal transduction (Rotation)

Selected Publications:

Liu, K.H., Niu, Y., Konish, M., Wu, Y., Du, H., Chung, H.S., Li, L., Boudsocq, M., McCormack, M., Maekawa, S., Ishida, T., Zhang, C., Shokat, K., Yanagisawa, S., and Sheen, J. 2017. Discovery of nitrate-CPK-NLP signaling in central nutrient-growth networks. Nature 545:311-316  PDF

Li L. and Sheen, J. 2016. Dynamic and diverse sugar signaling. Curr. Opin. Plant Biol. 33:116-125  PDF

Cheng, Z., Li, J.F., Niu, Y., Zhang, X.C., Woody, O.Z., Xiong, Y., Djonovic, S., Millet, Y., Bush, J., McConkey, B.J., Sheen, J., and Ausubel, F.M. 2015. Pathogen-secreted proteases activate a novel plant immune pathway. Nature 521:213-216  PDF

Xiong, Y. and Sheen, J. 2015. Novel links in the plant TOR signaling network. Curr. Opin. Plant Biol. 28:83-91  PDF

Sheen, J. 2014. Master regulators in plant glucose signaling networks. J. Plant Biol. 57:67-79  PDF

Xiong, Y. and Sheen, J. 2014. TOR signaling network in plant growth and metabolism. Plant Physiol. 164:499-512  PDF

Cheng, C., Gao, X., Feng, B., Sheen, J., Shan, L and He, P. 2013. Differential temperature operation of plant immune responses. Nature Commun. 4:2530 doi: 10.1038/ncomms3530  PDF

Gao, X., Chen, X., Lin W., Lu, D., Niu, Y., Li, L., Cheng, C., McCormack, M., Sheen, J., Shan, L. and He, P. 2013. Bifurcation of Arabidopsis NLR immune signaling via Ca2+-sensor protein kinases. PLOS Pathogens 9:e1003127  PDF

Boudsocq, M. and Sheen, J. 2013. CDPKs in immune and stress signaling. Trends in Plant Science. 18:30-40  PDF

Li, J.F., Chung, H.S., Niu, Y., Bush, J., McCormack, M. and Sheen, J. 2013. Comprehensive protein-based artificial microRNA screens for effective gene silencing in plants. Plant Cell 25:1507-1522  PDF

Li, J.F., Norville, E.J., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G.M. and Sheen, J. 2013. Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nature Biotechnology 31:688-691  PDF

Hwang, I., Sheen, J. and Müller, B. 2012. Cytokinin signaling networks. Annual Review of Plant Biology 63:353-380  PDF

Lee, H., Chah, O.K., Plotnikov, J. and Sheen, J. 2012. Stem Cell Signaling in Immunity and Development. CSHL Symposium on Quantitative Biology. Online doi:10.1101/sqb.2012.77.014837  PDF

Tena, G., Boudsocq, M. and Sheen, J. 2011. Protein kinase signaling networks in plant innate immunity. Curr. Opin. Plant Biol. 14:519-529 PDF

Lee, H., Chah, O.K. and Sheen, J. 2011. Stem-cell-triggered immunity through by CLV3p-FLS2 signaling. Nature 473:376-379 PDF  SUPP

Boudsocq, M., Willman, M., McCormack, M., Lee, H., Shan, L., He., P., Bush, J., Cheng, S.H., and Sheen, J. 2010. Differential innate immune signalling via Ca2+ sensor protein kinases. Nature 464:418-423 PDF  SUPP

Yoo, S.D., Cho, Y.H.,, and Sheen, J. 2009. Emerging connections in the ethylene-signaling network. Trends in Plant Science.14:270-279 PDF

Shan, L., He, P., Li, J., Heese, A., Peck, S.C., Nürnberger, T., Martin, G.B. and Sheen, J. 2008. Bacterial Effectors Target the Common Signaling Partner BAK1 to Disrupt Multiple MAMP Receptor Signaling Complexes and Impede Plant Immunity. Cell Host & Microbe 4:17-27  PDF  SUPP

Müller, B. and Sheen, J. 2008. Cytokinin and auxin interaction in root stem-cell specification during early embryogenesis. Nature 453:1094-1097  PDF  SUPP

Yoo, S-D., Cho Y-H., Tena G., Xiong. Y. and Sheen, J. 2008. Dual control of nuclear EIN3 by bifurcate MAPK cascades in C2H4 signalling. Nature 451:789-795  PDF  SUPP

Shan, L., He, P.,and Sheen, J. 2007. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host & Microbe 1:167-174 PDF SUPP

Baena-González, E., Rolland, F., Thevelein, J.M. and Sheen, J. 2007. A central integrator of transcription networks in plant stress and energy signalling. Nature 448:938-943 PDF SUPP

Yoo, S.D., Cho, Y.H.and Sheen, J. 2007. Arabidopsis Mesophyll Protoplasts: A versatile cell system for transient gene expression analysis. Nature Protocols 2:1565-1575. PMID 17585298 PDF SUPP

Cho, Y.-H., Yoo, S.-D., Sheen, J. 2006. Regulatory Functions of Nuclear Hexokinase1 Complex in Glucose Signaling. Cell 127:579-589 PDF SUPP.

Rolland, F., Baena-González, E., and Sheen, J. 2006. Sugar Sensing and Signaling in Plants: Conserved and Novel Mechanisms. Annual Review of Plant Biology 57:676-709 PDF

He, P., Shan, L., Lin, N.-C., Martin, G.B., Kemmerling, B., Nürnberger, T., and Sheen, J. 2006. Specific Bacterial Suppressors of MAMP Signaling Upstream of MAPKKK in Arabidopsis Innate Immunity. Cell 125:563-575 PDF SUPP.

Yanagisawa, S., Yoo, S.-D.and Sheen, J. 2003. Differential regulation of EIN3 stability by glucose and ethylene signaling in plants. Nature 425:521-525 PDF SUPP.

Moore, B., Zhou, L.,Rolland, F., Hall, Q., Cheng, W.-H., Liu, Y.-X., Hwang, I., Jones, T., and Sheen, J. 2003. Role of the Arabidopsis Glucose Sensor HXK1 in Nutrient, Light, and Hormonal Signaling. Science 300:332-336 PDF SUPP.

Asai, T., Tena, G., Plotnikova, J., Willmann, M., Chiu, W-L., Gomez-Gomez, L., Boller, T., Ausubel, F.M., and Sheen, J. 2002. MAP kinase signaling cascade in Arabidopsis innate immunity. Nature 415:977-983 PDF

Hwang,I. and Sheen, J. 2001. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature 27:413(6854):383-9 PDF

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